反应详情

温度

40 °C

1.5 ml of triethylamine, 1.6 ml of N,N-dimethylaniline and 2.3 ml of trimethylchlorosilane are added to a suspension of 2.0 g of 7-amino-3-(1-methyl-1H-tetrazol-5-ylthio)-methylceph-3-em-4-carboxylic acid in 60 ml of methylene chloride. The mixture is warmed to 40° C over the course of 20 minutes whilst stirring, and is then cooled to -10° C. A solution of 2-(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetic acid chloride in 20 ml of methylene chloride, which has been prepared in the manner described in Example 2 from 1.95 g of 2(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetic acid, is then added. The whole is stirred for 2 hours at 0°-25° C and poured into water, the mixture is adjusted to approx. pH 7 with sodium bicarbonate, extracted with methylene chloride and carefully acidified with concentrated hydrochloric acid and the precipitate formed is filtered off. The filter residue is washed with water and dried in vacuo at room temperature. The dried intermediate product is dissolved in 5 ml of trifluoroacetic acid whilst stirring and after 10 minutes the solution is concentrated in vacuo with repeated addition of toluene. The residue is stirred with 50 ml of methanol and filtered and the filtrate is adjusted to approx. pH 6 with triethylamine and left in a refrigerator for 2 hours. Filtration and washing of the filter residue with a little methanol and ether gives 7β-[2-(5-aminomethyl-2-thienyl)-acetylamino]-3-[(1-methyl-1H-tetrazol-5-ylthio)-methyl]-ceph-3-em-4-carboxylic acid; thin layer chromatogram (silica gel; acetonitrile/water, 4:1): Rf = 0.25; ultraviolet absorption spectrum (in 0.01 N aqueous sodium bicarbonate solution): λmax = 242 mμ (ε = 14,100); infrared absorption spectrum (in mineral oil): bands at 3.07μ, 5.64μ, 5.97μ, 6.26μ, 6.64μ etc.

1. temperatureis then cooled to -10° C
2. additionis then added
3. stirringThe whole is stirred for 2 hours at 0°-25° C
4. extractionextracted with methylene chloride
5. customthe precipitate formed
6. filtrationis filtered off
7. washThe filter residue is washed with water
8. customdried in vacuo at room temperature
9. dissolutionThe dried intermediate product is dissolved in 5 ml of trifluoroacetic acid
10. stirringwhilst stirring and after 10 minutes the solution
11. concentrationis concentrated in vacuo
12. additionaddition of toluene
13. stirringThe residue is stirred with 50 ml of methanol
14. filtrationfiltered
15. filtrationFiltration
16. washwashing of the filter residue with a little methanol and ether