反应详情

1-[3-(triphenylmethylthio)-2-methylpropanoyl]-L-proline tert.butyl ester (5 g.) is dissolved in a mixture of anisole (55 ml.) and trifluoroacetic acid (110 ml.). After one hour storage at room temperature, the solvents are removed in vacuo and the residue is applied to a column of silica gel equilibrated with benzene:acetic acid (75:25) and eluted with the same solvent. The fractions corresponding to the component with Rf 0.40 (TLC silica gel with same system) are pooled and concentrated to dryness to yield 1-[3-mercapto-2-methylpropanoyl]-L-proline. Rf 0.62 (silica gel, chloroform/methanol: acetic acid:water 50:40:10), identical to the compound of Example 34.

1. customthe solvents are removed in vacuo
2. washeluted with the same solvent
3. concentrationconcentrated to dryness