反应详情

温度

120 °C

X-Phos (0.96 g, 2.01 mmol, 0.3 eq.), Pd2(dba)3 (0.615 g, 0.672 mmol, 0.1 eq.), Cs2CO3 (4.38 g, 13.44 mmol, 2 eq.) were combined and flushed 10 min with Argon. To this mixture, a solution of (S)-3-(5,6,7,8-tetrahydro-pyrido[4,3-d]pyrimidin-4-yloxy)-pyrrolidine-1-carboxylic acid tert-butyl ester (intermediate 7) (2.15 g, 6.72 mmol) in dioxane (6 mL) and 5-bromo-2-methoxy-3-methylpyridine (1.76 g, 8.73 mmol) were added at rt and the reaction mixture was stirred at 120° C. for 2 h. The reaction was cooled down to rt, the reaction mixture filtered over Hyflo, AcOEt was added and the organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under vacuum. The residue was dissolved in dioxane (6 mL) and added to a glass vial containing 5-bromo-2-methoxy-3-methylpyridine (1.76 g, 8.73 mmol), X-Phos (0.96 g, 2.01 mmol), Pd2(dba)3 (0.615 g, 0.672 mmol), Cs2CO3 (4.38 g, 13.44 mmol). The vial was capped and the reaction mixture was stirred at 120° C. for 2 h. The reaction was cooled down to rt, the reaction mixture filtered over Hyflo, AcOEt was added and the organic layer was washed with brine, dried over Na2SO4, filtered and concentrated under vacuum. Purification by flash chromatography on silica gel (CH2Cl2 then TBME then TBME/MeOH 99/1 to 90/10) gave (S)-3-[6-(6-methoxy-5-methyl-pyridin-3-yl)-5,6,7,8-tetrahydro-pyrido[4,3-d]pyrimidin-4-yloxy]-pyrrolidine-1-carboxylic acid tert-butyl ester as a yellow foam (2.05 g, 69% yield). 1H NMR (400 MHz, DMSO-d6, 298K) δ ppm 1.35-1.44 (br.s., 9H) 2.07-2.23 (m, 2 H), 2.14 (s, 3 H), 2.87-2.93 (m, 2 H), 3.39-3.68 (m, 6 H), 3.81 (s, 3 H), 4.03-4.08 (m, 2 H), 5.56-5.63 (m, 1 H), 7.41-7.46 (m, 1 H), 7.67-7.73 (m, 1 H), 8.60 (s, 1 H). LCMS: [M+H]+=342.2, Rt(2)=0.94 min.

1. customflushed 10 min with Argon
2. temperatureThe reaction was cooled down to rt
3. filtrationthe reaction mixture filtered over Hyflo, AcOEt
4. additionwas added
5. washthe organic layer was washed with brine
6. dry with materialdried over Na2SO4
7. filtrationfiltered
8. concentrationconcentrated under vacuum
9. dissolutionThe residue was dissolved in dioxane (6 mL)
10. additionadded to a glass vial
11. customThe vial was capped
12. stirringthe reaction mixture was stirred at 120° C. for 2 h
13. temperatureThe reaction was cooled down to rt
14. filtrationthe reaction mixture filtered over Hyflo, AcOEt
15. additionwas added
16. washthe organic layer was washed with brine
17. dry with materialdried over Na2SO4
18. filtrationfiltered
19. concentrationconcentrated under vacuum
20. customPurification by flash chromatography on silica gel (CH2Cl2