反应详情

HATU (248 mg) and DIPEA (0.211 ml) were added to a flask containing 1-methyl-1H-1,2,4-triazole-3-carboxylic acid (51 mg) in DMF (1 ml). The reaction was stirred for 30 min under nitrogen. 6-(1H-Indol-4-yl)-1H-indazol-4-amine (50 mg) was added and the mixture was stirred for 3 h at RT under nitrogen. The reaction mixture was applied to an aminopropyl SPE cartridge (5 g) and left for 3 h. The column was washed with 10% methanol in DCM. The appropriate fractions were combined and the solvent was evaporated. The residue was dissolved in methanol:DMSO (1 ml, 1:1) and purified by mass directed preparative HPLC (Method A) to give title compound (37 mg).

1. stirringthe mixture was stirred for 3 h at RT under nitrogen
2. waitleft for 3 h
3. washThe column was washed with 10% methanol in DCM
4. customthe solvent was evaporated
5. dissolutionThe residue was dissolved in methanol
6. customDMSO (1 ml, 1:1) and purified by mass