HRID257720

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=c1ccn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID6031

未命名化合物

结构：O=P(O)(O)OC[C@H]1O[C@H](OP(=O)(O)O)[C@H](O)[C@@H](O)[C@@H]1O

HCID82400

alpha-D-Glucose 1,6-bis(dihydrogen phosphate)

C6H14O12P2

结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O

HCID439174

N-Acetylglucosamine cyclized form

C8H15NO6

结构：O=P([O-])([O-])OC1C[C@@H](O)[C@H](O)[C@@H](CO)O1

HCID86747384

未命名化合物

PRODUCTS

生成物

结构：CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O[C@H]2C[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@@H]1O

HCID15929800

未命名化合物

PROCEDURE

实验过程

2-Deoxyglucose 6-phosphate (2, 60 mg, 190 μmol), N-acetylglucosamine (6, 71 mg, 321 μmol), UDP (2 mg, 5 μmol), PEP (44 mg, 210 μmol), glucose 1, 6-diphosphate (0.1 mg) and bovine serum albumin (BSA, 5 mg) are dissolved in 10 ml of deoxygenated buffer. The enzymes are subsequently added:

WORKUP

后处理

additionThe enzymes are subsequently added