反应详情

The following tritiated drugs were used as radioligands for each of the receptors tested: [3H]-Spiperone 21-24 Ci/mmol for D2 receptors and [3H]-SCH23390 75-85 Ci/mmol for D1 receptors. The radioligands were incubated with various concentrations of competing drug and the appropriate membrane source for periods of time as follows: 75 minutes at room temperature for D2 receptors or 15 minutes at 37° C. for D1 receptors. Specific binding was defined using 1 μM butaclamol (D2) or 1 μM SCH23390 (D1). In addition, the D2 assays contained 30 nM ketaserin in order to block the binding of 3H-spiperone to 5HT2 receptors.

1. concentrationThe radioligands were incubated with various concentrations
2. waitthe appropriate membrane source for periods of time as follows
3. custom75 minutes
4. customat room temperature
5. customfor D2 receptors or 15 minutes
6. customat 37° C.