反应详情

(E)-4-Methyl-hept-2-enoic acid (21.3 mg, 0.15 mmol) was dissolved in DMF (350 μl), diisopropylethylamine was added (26 μl, 0.15 mmol) followed by HATU (57 mg, 0.15 mmol dissolved in 0.25 ml DMF). The mixture was stirred at room temperature for 15 minutes, then 2-methoxy-4-piperazin-1-yl-quinoline (36.4 mg, 0.15 mmol, dissolved in 1 ml DMF) was added and stirring was continued overnight. The mixture was evaporated under reduced pressure, the residue was dissolved in dichloromethane and the resulting solution was washed with 5% sodium bicarbonate solution, water, evaporated to dryness under reduced pressure. The residue was purified by preparative HPLC (gradient of water containing 0.1% NH3 and acetonitrile) to yield 21.1 mg (0.06 mmol, 39.8%) of a solid.

1. stirringstirring
2. waitwas continued overnight
3. customThe mixture was evaporated under reduced pressure
4. dissolutionthe residue was dissolved in dichloromethane
5. washthe resulting solution was washed with 5% sodium bicarbonate solution, water
6. customevaporated to dryness under reduced pressure
7. customThe residue was purified by preparative HPLC (gradient of water containing 0.1% NH3 and acetonitrile)