反应详情

N,N-diisopropylethylamine (0.033 ml) and acryloyl chloride (0.0154 ml) were added to a solution of 5-(quinolin-3-yl)-6,7,8,9-tetrahydropyrimido[5,4-b]indolizine-4,8-diamine (68 mg) obtained in Step 11 in chloroform (2.5 ml) under ice-cooling. After stirring at the same temperature for 15 minutes, the mixture was poured into a saturated aqueous sodium bicarbonate solution, followed by extraction with chloroform. The organic layer was dried over anhydrous sodium sulfate, and the solvent was then distilled off under reduced pressure. The resulting residue was purified by silica gel column chromatography (developing solvent: chloroform/methanol) to obtain the title compound as a yellow solid (35.4 mg).

1. temperaturecooling
2. extractionfollowed by extraction with chloroform
3. dry with materialThe organic layer was dried over anhydrous sodium sulfate
4. distillationthe solvent was then distilled off under reduced pressure
5. customThe resulting residue was purified by silica gel column chromatography (developing solvent: chloroform/methanol)