反应详情

SHA-Sepharose 4B was prepared by mixing 130 mg of (6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid (SHA--Z--NH2), dissolved in 30 mL 0.2M NaHCO3, with 6.5 g HCl washed CNBr activated Sepharose 4B (Pharmacia) overnight at room temperature. After the coupling reaction, 2 mL of 0.5M Tris, pH 8.5 were added and the gel slurry mixed at room temperature for 1 hour, and washed with water, 0.5M NaCl, and water again. The resulting SHA-Sepharose 4B was suspended in 30 mL of 20% ethanol, and stored at 4° C.

1. washwashed CNBr
2. customSepharose 4B (Pharmacia) overnight
3. customat room temperature
4. customAfter the coupling reaction, 2 mL of 0.5M Tris, pH 8.5
5. additionwere added
6. additionthe gel slurry mixed at room temperature for 1 hour
7. washwashed with water, 0.5M NaCl, and water again
8. customstored at 4° C.