Hydrochloric Acid → Salicylhydroxamic Acid

HRID 363161Notes

Reaction equation

HRID 363161 reaction equation
NCCCCCC([C:9]1[C:18](N)=[CH:17][CH:16]=[C:11]([C:12]([NH:14][OH:15])=[O:13])[C:10]=1[O:20]C)=O.Cl>C([O-])(O)=O.[Na+]>[C:12]([NH:14][OH:15])(=[O:13])[C:11]1[C:10](=[CH:9][CH:18]=[CH:17][CH:16]=1)[OH:20]

Participants

4

Reactant 2

HCID 313Hydrochloric AcidHCID 88168864(6-Aminohexanoyl)-4-amino-methylsalicylhydroxamic acid

Product 1

HCID 66644Salicylhydroxamic Acid

Reagents, catalysts & solvents 1

HCID 516892Sodium BicarbonateSolvent

Procedure

SHA-Sepharose 4B was prepared by mixing 130 mg of (6-aminohexanoyl)-4-amino-methylsalicylhydroxamic acid (SHA--Z--NH2), dissolved in 30 mL 0.2M NaHCO3, with 6.5 g HCl washed CNBr activated Sepharose 4B (Pharmacia) overnight at room temperature. After the coupling reaction, 2 mL of 0.5M Tris, pH 8.5 were added and the gel slurry mixed at room temperature for 1 hour, and washed with water, 0.5M NaCl, and water again. The resulting SHA-Sepharose 4B was suspended in 30 mL of 20% ethanol, and stored at 4° C.

Workup

  1. washwashed CNBr
  2. customSepharose 4B (Pharmacia) overnight
  3. customat room temperature
  4. customAfter the coupling reaction, 2 mL of 0.5M Tris, pH 8.5
  5. additionwere added
  6. additionthe gel slurry mixed at room temperature for 1 hour
  7. washwashed with water, 0.5M NaCl, and water again
  8. customstored at 4° C.

Sources & evidence

ORD record
ord-77cbea53db6a4633abda384b1f9b7866
Source dataset
uspto-grants-1999_02
Patent number
US05872224

Reaction notes

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