5'-Atp → HCID 178518213, compound

HRID 363286Notes

Reaction equation

HRID 363286 reaction equation
[P:1]([O:13][CH2:14][C@H:15]1[O:19][C@@H:18]([N:20]2[C:29]3[N:28]=[CH:27][N:26]=[C:24](N)[C:23]=3[N:22]=[CH:21]2)[C@H:17]([OH:30])[C@@H:16]1[OH:31])([O:4]P(OP(O)(O)=O)(O)=O)(=[O:3])[OH:2].[C@@H]1(N2C3N=CN=C(O)C=3N=C2)O[C@H](CO)[C@@H](O)[C@H]1[OH:34].S([O-])([O-])(=O)=O.[Mg+2]>C1(C)C(C)=CC=CC=1>[CH:27]1[NH:28][C:29]2[N:20]([C@@H:18]3[O:19][C@H:15]([CH2:14][O:13][P:1]([OH:2])([OH:4])=[O:3])[C@@H:16]([OH:31])[C@H:17]3[OH:30])[CH:21]=[N:22][C:23]=2[C:24](=[O:34])[N:26]=1

Participants

5

Reactant 3

HCID 59575'-AtpHCID 24083Sulfuric acid magnesium salt (1:1)HCID 178521066(2R,3S,4R,5R)-2-(hydroxymethyl)-5-(6-hydroxypurin-9-yl)oxolane-3,4-diol

Product 1

HCID 178518213HCID 178518213, compound

Reagents, catalysts & solvents 1

HCID 7237O-XyleneSolvent

Procedure

Escherichia coli cells containing the recombinant DNA collected in Example 2 (3) were suspended (cell concentration was 100 g wet cell weight/ml) in 100 mM Tris-hydrochloride buffer (pH 8.0) containing 10 mM ATP, 10 mM inosine and 5 mM magnesium sulfate. Xylene was added to the suspension in a concentration of 10 ml/l. After thoroughly stirring, the mixture was allowed to stand at 30° C. for an hour. The reaction solution was analyzed by HPLC and an amount of 51 -IMP in the reaction solution was quantitatively determined. Almost all transformants did not produce 5'-IMP but 5'-IMP-producing bacteria were obtained in a proportion of 1 out of 50,000 samples, The thus obtained 5'-IMP-producing bacteria are transformants carrying a recombinant DNA containing inosine guanosine-kinase gene.

Workup

  1. waitto stand at 30° C. for an hour

Sources & evidence

ORD record
ord-fd1c569cce7d430b8dd60d7fe1707974
Source dataset
uspto-grants-1999_02
Patent number
US05874272

Reaction notes

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