反应详情

温度

0 °C

Using Method C above, N-Boc-1-prop-2-ynylamino-propan-2-ol (0.13 g, 0.63 mmol) (Example 125A above) was coupled with (Z)-3-[(4-acetyl-1H-pyrrol-2-yl)methylene]-1,3-dihydro-5-fluoro-4-iodo-2H-indol-2-one (0.1 g, 0.25 mmol) (Example 90B) using (Ph3P)4Pd (30.0 mg) and Cul (5.0 mg) as catalyst in DMF (5 mL) and Et3N (5 mL) as solvent at 85° C. for 5 h. To the resulting compound in CH2Cl2 (6 mL) was added a 1:1 mixture of trifluoroacetic acid/CH2Cl2 (6 mL) and 3 drops of water at 0° C. and the mixture was stirred at 0° C. for 2 h. The mixture was then quenched with conc. NH4OH (6 mL) and diluted with EtOAc. The organic layer was washed with brine and dried with MgSO4. The resulting product was purified via flash column chromatography (10% MeOH in CH2Cl2). To the free base in methanol (2 mL) was added 4N HCl in dioxane (0.06 mL). Evaporation of solvent to dryness gave rac-(Z)-3-[(4-Acetyl-1H-pyrrol-2-yl)methylene]-1,3-dihydro-5-fluoro-4-[3-(2-hydroxy-propylamino)-1-propynyl]-2H-indol-2-one hydrochloride salt. (Yield 39 mg, 35%).

1. customThe mixture was then quenched with conc. NH4OH (6 mL)
2. additiondiluted with EtOAc
3. washThe organic layer was washed with brine
4. dry with materialdried with MgSO4
5. customThe resulting product was purified via flash column chromatography (10% MeOH in CH2Cl2)
6. additionTo the free base in methanol (2 mL) was added 4N HCl in dioxane (0.06 mL)
7. customEvaporation of solvent to dryness