反应详情

Synthetic L-histidyl-L-proline diketopiperazine was prepared by coupling benzyloxycarbonyl-L-histidine (about 20 mmol, ICN) with L-prolineamide (about 20 mmol, Vega-Fox) in the presence of hydroxybenzotriazole (about 40 mmol, Aldrich), N-ethylmorpholine (about 20 mmol, Aldrich) and dicyclohexylcarbodiimide (about 20 mmol, Pierce). The solution in about 175 ml of dimethylformamide was stirred in an ice-bath for about 3 hours and then overnight at room temperature.

1. customovernight
2. customat room temperature