反应详情

The culture broth obtained similarly to Example 1 was centrifuged to separate the cells therefrom. The cells were suspended in 200 ml of a 0.05 mol/l phosphate buffer solution adjusted in advance to pH 7.0 and the suspension was again centrifuged to clean the cells. This cleaning was repeated to give a total of three cycles. A 0.5 g portion of the cleaned cells was added to a 100 ml of solution containing 1.0 g of dihydrated calcium hydrogen cis-epoxysuccinate and 0.3 g of calcium carbonate as the substrate. The resultant mixture was adjusted to pH 8.0 and shaken at 35° C for 4 hours. The substrate was wholly used up and the reaction mixture was found to have produced calcium L(+)-tartrate. The amount of calcium L(+)-tartrate thus produced was 1.09 g. The yield was 98%. The resultant reaction mixture was incorporated with both 1.0 g of dihydrated calcium hydrogen cis-epoxysuccinate and 0.3 g of calcium carbonate thereto and was shaken at 35° C for 4 hours. The substrate present in the reaction mixture was wholly used up and converted into calcium L(+)-tartrate. The amount of the product was 2.18 g and the yield thereof based on the charged substrate was 98%. In this manner, 1.0 g portions of dihydrated calcium hydrogen cis-epoxysuccinate and 0.3 portions of calcium carbonate were added one each at a time to respective totals of 20.0 g and 6.0 g. Finally, the substrate thus successively added was wholly used up to produce the precipitate of calcium L(+)-tartrate. The amount of the product thus produced was 22.0 g and the yield thereof was 99 percent. The calcium L(+)-tartrate was suspended in about 200 ml of water. While the suspension was kept under agitation, 1N hydrochloric acid was added until the precipitate of calcium L(+)-tartrate was dissolved. The resultant liquid was centrifuged to remove the microbic cells therefrom. The remaining solution was passed through a column measuring 3 × 80 cm and packed with Amberlite IR 120 (H+ type cation-exchange resin) and the adsorbate was eluted with water. By concentrating to dryness the eluate under reduced pressure, there was obtained 15.7 g of L(+)-tartaric acid in the form of crystals. The yield was 89 percent and the optical purity was 100 percent.

1. customThe amount of the product thus produced
2. customthe yield
3. dissolutionwas dissolved
4. customto remove the microbic cells
5. washthe adsorbate was eluted with water
6. concentrationBy concentrating to dryness the eluate under reduced pressure