Ammonium Hydroxide → 6-Pentyltetrahydro-2H-pyran-2-one

A 15 1 bioreactor containing 10 1 of medium was seeded with 2×800 ml of an inoculum of Clostridium tyrobutyricum I-776. Strict anaerobic and sterile conditions were maintained. The pH was kept at 6.5, the temperature at 35° C., the stirring speed at 300/min; the initial concentration of glucose was 90 g/l. When the glucose was exhausted (48 h), the cells were gathered for centrifugation over 30 min at 4° C., for example at 10000 ×g. The humid mass of the cells was gathered and put into a 2 1 reactor under strictly sterile and anaerobic conditions. The volume was adjusted to a total of 1 1 with a buffer solution of pH 7. The medium contained about 90 g/l of dry matter. NH4OH was added to maintain a pH of 7, and a glucose solution of 500 g/l was also added, to adjust the glucose concentration to about 20 g/l. 2-Decen-5-olide was added semicontinuously. After 40 h, δ-decalactone was obtained in a concentration of 7 g/l, which corresponds to a conversion of 72%.

1. additionA 15 1 bioreactor containing 10 1 of medium
2. temperatureStrict anaerobic and sterile conditions were maintained
3. customat 35° C.
4. custom(48 h)
5. customwere gathered for centrifugation over 30 min at 4° C., for example at 10000 ×g
6. temperatureto maintain a pH of 7
7. concentrationthe glucose concentration to about 20 g/l
8. addition2-Decen-5-olide was added semicontinuously

ORD record

ord-ef0580bd11a840ccaaa4ae2c4db15cf5

Source dataset

uspto-grants-2000_02

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06025170