1,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride → Coumarin

HRID 377417Notes

Reaction equation

HRID 377417 reaction equation
C([C:4]1[C:5](=[O:15])[O:6][C:7]2[C:12]([CH:13]=1)=[CH:11][CH:10]=[C:9](O)[CH:8]=2)(O)=O.Cl.C(N=C=NCCCN(C)C)C>>[O:6]1[C:7]2[C:12](=[CH:11][CH:10]=[CH:9][CH:8]=2)[CH:13]=[CH:4][C:5]1=[O:15]

Participants

3

Reactant 2

HCID 27239391,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochlorideHCID 53377573-Carboxy-7-hydroxycoumarin2×

Product 1

HCID 323Coumarin

Conditions

Temperature
4 °C

Procedure

Antibody which binds specifically to atherosclerotic plaque (1 m mole) is dialyzed st 4° C. against a buffer solution of 0.01M PBS, pH 6.8 overnight. To this solution is added 50 nmole of 3-carboxy-7-hydroxycoumarin. The solution is added 50 mnole of 3-carboxy-7-hydroxycoumarin. The solution is cooled in an ice bath and added with 50 nmole of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride. After addition the mixture was stirred at 4° C. for one hour and chromatographed on a 2.5 c 50 cm column of SEPHAEX® G-50. The absorbance of the conjugate is monitored at 345 nm to yield a solution of coumarin labeled antibody.

Workup

  1. customis dialyzed st 4° C. against a buffer solution of 0.01M PBS, pH 6.8 overnight
  2. temperatureThe solution is cooled in an ice bath
  3. additionAfter addition the mixture
  4. customchromatographed on a 2.5 c 50 cm column of SEPHAEX® G-50

Sources & evidence

ORD record
ord-09200631fa8545d7a7b8258a5cd5a6aa
Source dataset
uspto-grants-2000_02
Patent number
US06025477

Reaction notes

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