反应详情

To a solution of N-[(3-chloropyrazin-2-yl)-(2-phenylquinolin-7-yl)-methyl]-2,2-dimethylpropionamide (264 mg, 0.612 mmol) in THF (3 mL), cooled to 0° C., KOtBu (800 μL, 1 M, 0.796 mmol) was added, the cooling bath was removed, and the reaction mixture stirred at ambient temperature for 30 min, under N2. THF was removed in vacuo, POCl3 (25 mL, 42 g, 0.273 mol) was added to the residue, and the reaction mixture was vortexed at 70° C., under N2, for 5 d. POCl3 was evaporated (min. 2 h on high-vacuum), a cold solution of NH3/i-PrOH (2M, 10 mL) was added, the suspension was filtered, and the solid was washed several times with i-PrOH. The filtrate was concentrated, extracted with CH2Cl2 (3×30 mL), washed with brine (50 mL), dried over MgSO4, filtered, and concentrated in vacuo. The crude material was dissolved in CH2Cl2, adsorbed onto Hydromatrix, and purified by chromatography on silica gel [Jones Flashmaster, 5 g/25 mL cartridge, eluting with EtOAc:CH2Cl2 2%→5%], yielding the title compound, as a yellow solid; 1H NMR (CDCl3, 400 MHz) δ 1.26 (s, 9H), 7.33 (d, J=4.8 Hz, 1H), 7.44-7.50 (m, 1H), 7.50-7.58 (m, 2H), 7.87-7.94 (m, 4H), 8.17-8.22 (m, 2H), 8.24-8.30 (m, 1H), 8.51 (s, 1H); MS (ES+): m/z 412.9/414.9 (100/38) [MH+]; HPLC: tR=4.3 min (OpenLynx, polar—5 min).

1. customthe cooling bath was removed
2. additionwas added to the residue
3. waitthe reaction mixture was vortexed at 70° C., under N2, for 5 d
4. customPOCl3 was evaporated (min. 2 h on high-vacuum)
5. additiona cold solution of NH3/i-PrOH (2M, 10 mL) was added
6. filtrationthe suspension was filtered
7. washthe solid was washed several times with i-PrOH
8. concentrationThe filtrate was concentrated
9. extractionextracted with CH2Cl2 (3×30 mL)
10. washwashed with brine (50 mL)
11. dry with materialdried over MgSO4
12. filtrationfiltered
13. concentrationconcentrated in vacuo
14. dissolutionThe crude material was dissolved in CH2Cl2
15. custompurified by chromatography on silica gel [Jones Flashmaster, 5 g/25 mL cartridge, eluting with EtOAc:CH2Cl2 2%→5%]