Hydrochloric Acid → HCID 22823184, compound

HRID 414164Notes

Reaction equation

HRID 414164 reaction equation
[P:1]([O:5][CH2:6][C@H:7]1[O:11][C@@H:10]([N:12]2[C:21]3[N:20]=[CH:19][N:18]=[C:16]([NH2:17])[C:15]=3[N:14]=[C:13]2[NH:22][CH2:23][CH2:24][NH2:25])[C@H:9]([OH:26])[C@@H:8]1[OH:27])([OH:4])([OH:3])=[O:2].[OH-].[Na+].C(=O)(O)[O-].[Na+].F[C:36]1[CH:41]=[CH:40][C:39]([N+:42]([O-:44])=[O:43])=[CH:38][C:37]=1[N+:45]([O-:47])=[O:46].Cl>O.C(O)C.CC(C)=O>[P:1]([O:5][CH2:6][C@H:7]1[O:11][C@@H:10]([N:12]2[C:21]3[N:20]=[CH:19][N:18]=[C:16]([NH2:17])[C:15]=3[N:14]=[C:13]2[NH:22][CH2:23][CH2:24][NH:25][C:40]2[CH:41]=[CH:36][C:37]([N+:45]([O-:47])=[O:46])=[CH:38][C:39]=2[N+:42]([O-:44])=[O:43])[C@H:9]([OH:26])[C@@H:8]1[OH:27])([OH:3])([OH:4])=[O:2]

Participants

9

Reactant 5

HCID 313Hydrochloric AcidHCID 6264DinitrofluorobenzeneHCID 14798Sodium HydroxideHCID 516892Sodium BicarbonateHCID 228231868-[(2-Aminoethyl)amino]adenosine 5'-(dihydrogen phosphate)

Product 1

HCID 22823184HCID 22823184, compound

Reagents, catalysts & solvents 3

HCID 180AcetoneSolventHCID 702EthanolSolventHCID 962WaterSolvent

Procedure

8-(2-aminoethyl)aminoadenosine-5'-monophosphate from Part A of this Example (0.64 mmol) was dissolved in 20 ml water by addition of sodium hydroxide to pH 8.0. Then 168 mg sodium bicarbonate was added, followed by 0.2 ml 1-fluoro-4-dinitrobenzene (1.58 mmol dissolved in 2 ml ethanol). The reaction was stirred for 18 hr at room temperature and then 0.1 ml 1-fluoro-2,4-dinitrobenzene in 1 ml ethanol was added. After stirring for an additional 4 hrs, the mixture was adjusted to pH 2.0 with hydrochloric acid and poured into 200 ml cold acetone (-10° C.). The yellow precipitate which formed was collected by filtration, dissolved in 200 ml water and passed into a 2.5×45 cm column of DEAE-cellulose in the bicarbonate form. The chromatogram was developed with a linear salt gradient generated with 2 liters water and 2 liters 0.7M ammonium carbonate. A peak of yellow material with an absorption maximum at 275 nm eluted between 2 and 3 liters of the gradient. Ammonium bicarbonate was removed from this material by evaporation under vacuum and the yield of the desired intermediate was 37 percent. Optical absorption maxima measured in 0.02N hydrochloric acid occurred at 275 and 363 nm and the millimolar extinction coefficients were 21.8 and 15.5, respectively. Further analyses showed 1.07 phosphate residue per ribose residue.

Workup

  1. stirringAfter stirring for an additional 4 hrs
  2. customThe yellow precipitate which formed
  3. filtrationwas collected by filtration
  4. dissolutiondissolved in 200 ml water
  5. washA peak of yellow material with an absorption maximum at 275 nm eluted between 2 and 3 liters of the gradient
  6. customAmmonium bicarbonate was removed from this material by evaporation under vacuum
  7. customOptical absorption maxima

Sources & evidence

ORD record
ord-beb35abde30142b1b6f9675f5cf1228f
Source dataset
uspto-grants-1988_12
Patent number
US04791055

Reaction notes

Loading…