反应详情

温度

0 °C

The intermediate (b) was prepared as described in Synthesis 2001, 11, 1719-1730. (1S,2R,3S,4R)-3-(Methoxycarbonyl)bicyclo[2.2.1]hept-5-ene-2-carboxylic acid (8.94 g, 45.57 mmol) was dissolved in anhydrous tetrahydrofuran (200 mL). The flask was degassed and backfilled with nitrogen and the mixture was cooled to 0° C. Triethylamine (19.2 mL, 136.7 mmol) was added followed by the dropwise addition of ethyl chloroformate (8.69 mL, 91.1 mmol) with vigorous stirring. Immediate precipitation was observed. The mixture was stirred at 0° C. for 1 h. Sodium azide (8.89 g, 136.7 mmol) was dissolved in water (60 mL) and added to the reaction mixture at 0° C. The mixture was stirred at 0° C. for 1 h. The ice bath was removed. The mixture was warmed to 25° C. and continued to stir for 2 h. The mixture was poured into water (400 mL) and the product extracted into ethyl acetate (400 mL). The organic layer was further washed with half-saturated aqueous sodium bicarbonate solution (2×200 mL), saturated aqueous brine solution (2×200 mL), dried over magnesium sulfate, filtered, and concentrated in vacuo to afford a slightly brown oil. The oil was dissolved in anhydrous benzene (100 mL) and refluxed while stirring under nitrogen for 2 h. Upon cooling to 25° C. the solution was concentrated in vacuo to afford a slightly brown oil. The oil was dissolved in dichloromethane (60 mL) and benzyl alcohol (5.19 mL, 50.13 mmol) was added followed by triethylamine (12.81 mL, 91.14 mmol). The mixture was refluxed under nitrogen for 16 h. Upon cooling to 25° C. the solution was concentrated in vacuo to afford a golden oil. Purification by flash column chromatography (Merck silica gel 60, 40-63 μm, 10% ethyl acetate in hexanes) afforded the desired product, methyl (1R,2S,3R,4S)-3-{[(benzyloxy)carbonyl]amino}bicyclo[2.2.1]hept-5-ene-2-carboxylate (10.1 g, 33.55 mmol, 74%), as a clear oil. 1H NMR (400 MHz, CDCl3) δ: 1.38 (1H, d, J=8.7 Hz), 1.50 (1H, d, J=8.4 Hz), 3.10 (2H, s), 3.21 (1H, d, J=8.8 Hz), 3.53 (3H, s), 4.59-4.64 (1H, m), 5.07 (2H, q, J=13.0 Hz), 5.29 (1H, d, J=8.3 Hz), 6.15-6.17 (1H, m), 6.37-6.38 (1H, m), 7.27-7.36 (5H, m). LC-MS (ESI) calcd for C17H19NO4 301.13, found 258.1 (100%), 302.2 [M+H+] (70%), 603.5 [2M+H+] (20%).

1. customas described in Synthesis 2001, 11, 1719-1730
2. customThe flask was degassed
3. customImmediate precipitation
4. additionadded to the reaction mixture at 0° C
5. stirringThe mixture was stirred at 0° C. for 1 h
6. customThe ice bath was removed
7. temperatureThe mixture was warmed to 25° C.
8. stirringto stir for 2 h
9. additionThe mixture was poured into water (400 mL)
10. extractionthe product extracted into ethyl acetate (400 mL)
11. washThe organic layer was further washed with half-saturated aqueous sodium bicarbonate solution (2×200 mL), saturated aqueous brine solution (2×200 mL)
12. dry with materialdried over magnesium sulfate
13. filtrationfiltered
14. concentrationconcentrated in vacuo
15. customto afford a slightly brown oil
16. temperaturerefluxed
17. stirringwhile stirring under nitrogen for 2 h
18. temperatureUpon cooling to 25° C. the solution
19. concentrationwas concentrated in vacuo
20. customto afford a slightly brown oil
21. temperatureThe mixture was refluxed under nitrogen for 16 h
22. temperatureUpon cooling to 25° C. the solution
23. concentrationwas concentrated in vacuo
24. customto afford a golden oil
25. customPurification by flash column chromatography (Merck silica gel 60, 40-63 μm, 10% ethyl acetate in hexanes)