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HRID427199

反应详情

EQUATION

反应方程式

HRID 427199 的结构方程式

REACTANTS

反应物

4
结构：Cl
HCID313

Hydrochloric Acid

ClH

结构：N[C@@H](Cc1ccccc1)C(=O)O
HCID6140

L-Phenylalanine

C9H11NO2

3 次

结构：CC(C)C[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccccc1)NC(=O)OC(C)(C)C)C(=O)N[C@@H](Cc1ccccc1)C(=O)O
HCID132822

L-Phenylalanine, N-[(1,1-dimethylethoxy)carbonyl]-L-phenylalanyl-L-leucyl-L-phenylalanyl-L-leucyl-

C44H59N5O8

结构：O=Cc1ccccc1C=O.OCCS
HCID88526415

未命名化合物

PRODUCTS

生成物

1
结构：O=Cc1ccccc1C=O
HCID4807

1,2-Benzenedicarboxaldehyde

C8H6O2

AUXILIARIES

试剂、催化剂与溶剂

1
结构：CO
溶剂HCID887

Methanol

CH4O

PROCEDURE

实验过程

Four aliquots of the conjugate were hydrolyzed with concentrated HCl at 11520 C. for 20 hours and analyzed for phenylalanine. The phenylalanine was quantified as the o-phthalaldehyde/2-mercaptoethanol derivative using reverse phase HPLC with a fluorescence detector (HPLC conditions as Example 24 except the eluent gradient was 12% to 80% methanol over 20 min. and then held at 80% for 5 min.). The o-phthalaldehyde derivative was prepared as given in Example 24. For a 25 μL aliquot of the conjugate, 26 nmoles phenylalanine were found. This corresponds to 0.26 pmoles BOCPheLeuPheLeuPhe on the conjugate.

WORKUP

后处理

  1. customover 20 min.
  2. waitheld at 80% for 5 min.