反应详情

温度

60 °C

Frozen flocculated cells are immersed in water or a glucose syrup. The cell mass is allowed to thaw and is gently crushed by mechanical means or stirring to give particles of approximately uniform size. This slurry is then subjected to vacuum conditions for approximately 30 minutes to remove occluded gases from the cell particles. A jacketed column of 1 inch diameter is partially filled with water or glucose syrup and the degassed slurry is then poured into the column. The packed column is heated to 60° C. and a 2 M solution of glucose having a pH of 8.0 and containing 0.004 M MgCl2.6H2O is passed through the column at flow rates which are regulated to give the degree of glucose conversion desired. Conversions ranging up to 50 percent are attainable by this process.

1. temperatureFrozen
2. customis gently crushed by mechanical means
3. customto give particles of approximately uniform size
4. customto remove occluded gases from the cell particles
5. additionA jacketed column of 1 inch diameter is partially filled with water or glucose syrup
6. additionthe degassed slurry is then poured into the column
7. additiona 2 M solution of glucose having a pH of 8.0 and containing 0.004 M MgCl2.6H2O