Ethanol → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 439285Notes

Reaction equation

HRID 439285 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4].[CH2:11]([OH:13])C>>[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:13])[OH:10])[OH:8])[OH:6])[OH:4]

Participants

3

Reactant 2

HCID 702Ethanol2×HCID 644160aldehydo-D-Xylose2×

Product 1

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Procedure

The results presented in FIG. 2 show that in contrast to the control strain containing the shuttle vector alone (CP4 pZB186!), the recombinant containing the added xylose isomerase, xylulokinase, transaldolase and transketolase genes demonstrated growth on and ethanol production from xylose as a carbon source. The recombinant strain produces ethanol from glucose as efficiently as the control strain at 94% of theoretical yield. The recombinant strain additionally produces ethanol from xylose at 84% of theoretical yield in 79 hours. Furthermore, in the combined presence of glucose and xylose, the recombinant strain ferments both sugars simultaneously to ethanol at 88% of theoretical yield within 48 hours, thus providing the foundation for advanced process designs with cofermentation of mixed-sugar feedstocks.

Workup

  1. additioncontaining the shuttle vector alone (CP4 pZB186!)

Sources & evidence

ORD record
ord-a3e1d7a1f10d4c329d0adbfdff4e9569
Source dataset
uspto-grants-1996_05
Patent number
US05514583

Reaction notes

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