Triethylamine → HCID 14558097, compound

Triethylamine (13.4 ml, 100 mmol) was added dropwise to a stirred ice-cooled mixture of 1,2,4-triazole (6.22 g, 90 mmol) and phosphorous oxychloride (1.89 ml, 20 mmol) in 50 ml of anhydrous acetonitrile. To the resulting suspension the solution of 2',3',5'-tri-O-Benzoyl-6-methyl uridine (5.7 g, 10 mmol) in 30 ml of acetonitrile was added dropwise and the reaction mixture was stirred for 4 hours at room temperature. Then it was concentrated in vacuo to minimal volume (not to dryness). The residue was dissolved in chloroform and washed with water, saturated aq sodium bicarbonate and brine. The organic layer was dried over sodium sulfate and the solvent was removed in vacuo. The residue was dissolved in 100 ml of 1,4-dioxane and treated with 50 mL of 29% aq NH4OH overnight. The solvents were removed in vacuo. The residue was dissolved in the mixture of pyridine (60 mL) and methanol (10 mL), cooled to -15° C. and ice-cooled 2M aq solution of sodium hydroxide was added under stirring. The reaction mixture was stirred at -10° to -15° C. for additional 30 minutes and then neutralized to pH 7 with Dowex 50 (Py+). The resin was filtered off and washed with 200 mL of the mixture H2O--Py (4:1). The combined mother liquor and washings were evaporated to dryness. The residue was crystallized from aq methanol to give 1.6 g (62%) of 6-methyl cytidine.

1. temperaturecooled
2. concentrationThen it was concentrated in vacuo to minimal volume (not to dryness)
3. dissolutionThe residue was dissolved in chloroform
4. washwashed with water, saturated aq sodium bicarbonate and brine
5. dry with materialThe organic layer was dried over sodium sulfate
6. customthe solvent was removed in vacuo
7. dissolutionThe residue was dissolved in 100 ml of 1,4-dioxane
8. additiontreated with 50 mL of 29% aq NH4OH overnight
9. customThe solvents were removed in vacuo
10. dissolutionThe residue was dissolved in the mixture of pyridine (60 mL) and methanol (10 mL)
11. temperaturecooled to -15° C.
12. temperatureice-cooled 2M aq solution of sodium hydroxide
13. additionwas added
14. stirringunder stirring
15. stirringThe reaction mixture was stirred at -10° to -15° C. for additional 30 minutes
16. filtrationThe resin was filtered off
17. washwashed with 200 mL of the mixture H2O--Py (4:1)
18. customThe combined mother liquor and washings were evaporated to dryness
19. customThe residue was crystallized from aq methanol

ORD record

ord-4d647574825b4ab38d9cb157af89533c

Source dataset

uspto-grants-1999_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05891684