反应详情

N-[4-[[(2,4-Diamino-5-methylpyrido[2,3-d]pyrimidin-6-yl)methyl]ethylamino]benzoyl-L-glutamic acid (21, 5-Methyl-5-deaza-10-ethylaminopterin) was prepared by saponification of 19 as described for the conversion of 18 to 20; yield 97% (56 mg from 60 mg, 0.109 mmol), of 19·0.6H2O, homogeneous by HPLC. Spectral data: mass, m/e 482 (M+1)+ ; UV, 0.1N HCl, 228 (40.8), 313 (17.1; pH 7, 227 (39.2), 308 (30.2); 0.1N NaOH, 228 (36.0), 308 (29.0).