HRID467265

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=c1ccn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H]2O)c(=O)[nH]1

HCID8629

Uridine diphosphate glucose

C15H24N2O17P2

HCID24247

Perchloric acid

HClO4

结构：O=P(O)(O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O

HCID65533

alpha-D-Glucose 1-phosphate

C6H13O9P

HCID93573

Tromethamine hydrochloride

C4H12ClNO3

HCID5360315

Magnesium Chloride

Cl2Mg

结构：O=P([O-])([O-])OP(=O)([O-])OP(=O)([O-])[O-].[N-]=[N+]=N[C@H]1C[C@H](n2ccc(=O)[nH]c2=O)O[C@@H]1CO

HCID131717599

未命名化合物

PRODUCTS

生成物

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)OP(=O)(O)OP(=O)(O)O.[N-]=[N+]=N[C@H]1C[C@H](n2ccc(=O)[nH]c2=O)O[C@@H]1CO

HCID131717598

未命名化合物

PROCEDURE

实验过程

To 150 milligrams of AzddU-triphosphate was added 400 milligrams of glucose-1-phosphate, 6 millimole of MgCl2, 0.70 millimole of Tris-HCl pH 7.6, bacterial UDPG-pyrophosphorylase and inorganic pyrophosphatase. After 4 hours at room temperature, the reaction was stopped with perchloric acid (7N). After 30 minutes in ice, samples were centrifuged and the supernatant was neutralized with base. After another 30 minutes in ice, samples were recentrifuged and AzddU-5'-diphosphoglucose was purified by column chromatography.

WORKUP

后处理

waitAfter 30 minutes in ice
waitAfter another 30 minutes in ice
customAzddU-5'-diphosphoglucose was purified by column chromatography

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-3cfdeae288a2409fb130096d830f42b3
来源数据集: uspto-grants-1992_10
DOI: 10.6084/m9.figshare.5104873.v1
专利: US05159067
来源说明: uspto-grants-1992_10

反应 SMILES

[CH2:1]1[C@H:5]([N:6]2[C:12](=[O:13])[NH:11][C:9](=[O:10])[CH:8]=[CH:7]2)[O:4][C@H:3]([CH2:14][OH:15])[C@H:2]1[N:16]=[N+:17]=[N-:18].[O-:19][P:20]([O:23][P:24]([O:27]P([O-])([O-])=O)([O-:26])=[O:25])(=[O:22])[O-:21].[CH2:32]([OH:47])[C@H:33]1[O:38][C@H:37](OP(O)(O)=O)[C@H:36]([OH:44])[C@@H:35]([OH:45])[C@@H:34]1[OH:46].[Mg+2].[Cl-].[Cl-].C(O)C(N)(CO)CO.Cl.C1C(=O)NC(=O)N([C@@H]2O[C@H](COP(OP(O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)(O)=O)(O)=O)[C@@H](O)[C@H]2O)C=1.Cl(O)(=O)(=O)=O>>[N:16]([C@@H:2]1[C@@H:3]([CH2:14][OH:15])[O:4][C@@H:5]([N:6]2[CH:7]=[CH:8][C:9](=[O:10])[NH:11][C:12]2=[O:13])[CH2:1]1)=[N+:17]=[N-:18].[P:24]([O:27][C@H:33]([CH2:32][OH:47])[C@@H:34]([OH:46])[C@H:35]([OH:45])[C@@H:36]([OH:44])[CH:37]=[O:38])([O:23][P:20]([OH:21])([OH:19])=[O:22])([OH:26])=[O:25]