Hydrochloric Acid → Phosphate Buffered Saline

HRID 481702Notes

Reaction equation

HRID 481702 reaction equation
[Cl-:1].[K+:2].[P:3]([O-:7])([O-:6])([OH:5])=[O:4].[Na+:8].[Na+].[P:10]([O-:14])([OH:13])([OH:12])=[O:11].[K+].[N-]=[N+]=[N-].[Na+].Cl>O>[OH:5][P:3]([O-:7])([OH:6])=[O:4].[OH:12][P:10]([O-:14])([O-:13])=[O:11].[Na+:8].[Na+:8].[Na+:8].[Cl-:1].[Cl-:1].[K+:2].[K+:2]

Participants

7

Reactant 5

HCID 313Hydrochloric AcidHCID 4873Potassium ChlorideHCID 24203Sodium Phosphate, DibasicHCID 33557Sodium AzideHCID 516951Monobasic potassium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

Dulbecco's phosphate buffered saline (DPBS) is prepared by first dissolving, sequentially, about 160 g sodium chloride NaCl), about 4 grams potassium chloride (KCl), about 23 grams disodium hydrogen orthophosphate (Na2HPO4), about 4 grams potassium dihydrogen orthophosphate (KH2PO4), and about 10 grams sodium azide in a liter of reverse-osmosis (MilliQ) water. Then, the pH is adjusted to about 7.3 by adding appropriate amounts of HCl. Finally, the buffer solution is diluted to 1:20 (50 ml buffer solution with 950 ml reverse-osmosis water), and allowed to degas either in a screw-capped container overnight or under vacuum.

Workup

  1. customto degas either in a screw-capped container overnight or under vacuum

Sources & evidence

ORD record
ord-f7d06aaadbea43349f4cc150feb7e9ba
Source dataset
uspto-grants-1998_08
Patent number
US05789461

Reaction notes

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