反应详情

A mixture trans-3-hydroxy-(L)-proline (Acros, 5.0 g, 38 mmol), benzyl alcohol (8.0 mL, 76 mmol) and pTSA (7.4 g, 38 mmol) in 30 mL of benzene was refluxed overnight while water was removed using a Dean-Stark apparatus. The reaction mixture was cooled to rt, and was concentrated in vacuo to dryness. The residue was dissolved in CH2Cl2 (50 mL) and TEA (32 mL, 0.23 mol) and chlorotrimethylsilane (19 mL, 0.15 mol) were added. After heating the solution at 70° C. for 1 h, the reaction mixture was cooled to 0° C., and MeOH (3.1 mL, 76 mmol) was added. After stirring at rt for 1 h, a solution of trityl chloride (16 g, 57 mmol) in 50 mL of CH2Cl2 followed by TEA (8.0 mL, 57 mmol) were added. After stirring at rt for 3 days, the reaction mixture was diluted with 150 mL of EtOAc, and the precipitates were removed by filtering through celite. The filtrate was concentrated in vacuo to dryness, and the residue was stirred with K2CO3 (2.2 g) in MeOH (100 mL) for 5 h at rt. The reaction mixture was diluted with THF (100 mL), and a solution of KF (8.8 g) in 40 mL of water was added. After stirring at rt overnight, the reaction mixture was cooled to 0° C., diluted with EtOAc (100 mL), and neutralized by careful addition of 0.5 M aqueous sodium bisulfate. The reaction mixture was partitioned in water and EtOAc, and the product was extracted with EtOAc (3×). The combined extracts were dried over anhydrous MgSO4, filtered and concentrated in vacuo to dryness. The residue was purified by flash column chromatography on silica gel eluted with 4:1 to 1:4 hexane/Et2O to afford N-trityl-3(S)-hydroxy-(L)-proline, benzyl ester (8.8 g, 49%).

1. customwas removed
2. concentrationwas concentrated in vacuo to dryness
3. dissolutionThe residue was dissolved in CH2Cl2 (50 mL)
4. temperatureAfter heating the solution at 70° C. for 1 h
5. temperaturethe reaction mixture was cooled to 0° C.
6. additionwere added
7. stirringAfter stirring at rt for 3 days
8. customthe precipitates were removed
9. filtrationby filtering through celite
10. concentrationThe filtrate was concentrated in vacuo to dryness
11. stirringthe residue was stirred with K2CO3 (2.2 g) in MeOH (100 mL) for 5 h at rt
12. additionThe reaction mixture was diluted with THF (100 mL)
13. additiona solution of KF (8.8 g) in 40 mL of water was added
14. stirringAfter stirring at rt overnight
15. temperaturethe reaction mixture was cooled to 0° C.
16. additiondiluted with EtOAc (100 mL)
17. additionneutralized by careful addition of 0.5 M aqueous sodium bisulfate
18. customThe reaction mixture was partitioned in water
19. extractionEtOAc, and the product was extracted with EtOAc (3×)
20. dry with materialThe combined extracts were dried over anhydrous MgSO4
21. filtrationfiltered
22. concentrationconcentrated in vacuo to dryness
23. customThe residue was purified by flash column chromatography on silica gel
24. washeluted with 4:1 to 1:4 hexane/Et2O