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0.5 mL aliquots of the membrane preparation were incubated with unlabeled drugs, and 0.15 nM [3H]spiperone in a final volume of 1 mL containing 50 mM Tris HCl, 120 mM NaCl and 1 mM MgCl2 (pH 7.7). Nonspecific binding was measured in the presence of 100 nM (+)-butaclamol. After 15 minutes of incubation at 37° C., samples were filtered rapidly through Whatman GF/C glass filters under negative pressure, and washed three times with ice-cold binding buffer (5 mL).

1. custom0.5 mL aliquots of the membrane preparation
2. filtrationsamples were filtered rapidly through Whatman GF/C glass filters under negative pressure
3. washwashed three times with ice-cold binding buffer (5 mL)