Sorbosone, L- → L-(-)-Sorbose

HRID 519251Notes

Reaction equation

HRID 519251 reaction equation
[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([CH:7]=[O:8])=[O:6]>P([O-])([O-])([O-])=O.[K+].[K+].[K+]>[OH:9][C:4]1[C@@H:3]([C@@H:2]([OH:11])[CH2:1][OH:12])[O:10][C:7](=[O:8])[C:5]=1[OH:6].[CH2:1]([OH:12])[C@H:2]([OH:11])[C@@H:3]([OH:10])[C@H:4]([OH:9])[C:5]([CH:7]=[O:8])=[O:6].[OH:8][CH2:7][C:5]([C@H:4]([C@@H:3]([C@H:2]([CH2:1][OH:12])[OH:11])[OH:10])[OH:9])=[O:6]

Participants

5

Reactant 1

HCID 10921067Sorbosone, L-

Product 3

HCID 6904L-(-)-SorboseYield 2%HCID 10921067Sorbosone, L-Yield 1%HCID 54670067L-Ascorbic Acid

Reagents, catalysts & solvents 1

HCID 62657Potassium Phosphate, TribasicSolvent

Procedure

The reaction (200 μl) with the membrane fraction (100 μl) was carried out in 50 mM potassium phosphate buffer (pH7.0), 30° C. with shaking at 220 rpm for 15 h. The substrates tested were L-sorbosone (1% final concentration) and L-sorbose (2% final concentration). The final protein concentration used in the reaction was 1.5 mg/ml. At the end of the incubation period, 680 mg/l and 10 mg/l of Vitamin C had been produced from 1% L-sorbosone and 2% L-sorbose, respectively.

Workup

  1. customThe reaction (200 μl) with the membrane fraction (100 μl)
  2. concentrationconcentration) and L-sorbose (2% final
  3. concentrationconcentration)
  4. concentrationThe final protein concentration

Sources & evidence

ORD record
ord-0d416f2bfa0341b9861c7740c3ccefc6
Source dataset
uspto-grants-2015_08
Patent number
US09115378B2

Reaction notes

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