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HRID612389

反应详情

EQUATION

反应方程式

HRID 612389 的结构方程式

REACTANTS

反应物

6
结构：O=S(=O)(F)Cc1ccccc1
HCID4784

Phenylmethanesulfonyl fluoride

C7H7FO2S

结构：O=S(=O)(O)CCN1CCN(CCO)CC1
HCID23831

(4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

C8H18N2O4S

结构：O[C@H](CS)[C@H](O)CS
HCID446094

Dithiothreitol

C4H10O2S2

结构：Cl.NCCCNCCCCNCCCN
HCID5351186

N~1~,N~4~-Bis(3-aminopropyl)butane-1,4-diamine--hydrogen chloride (1/1)

C10H27ClN4

结构：[Cl-].[Cl-].[Mg+2]
HCID5360315

Magnesium Chloride

Cl2Mg

结构：Cl.NCCCNCCCCNCCCNC1(CO)O[C@H](CO)[C@@H](O)[C@@H]1O
HCID86611429

未命名化合物

PRODUCTS

生成物

2
结构：NCCCNCCCCNCCCN
HCID1103

N1,N4-Bis(3-aminopropyl)-1,4-butanediamine

C10H26N4

结构：NCCCNCCCCNCCCNC1(CO)O[C@H](CO)[C@@H](O)[C@@H]1O
HCID86611428

未命名化合物

PROCEDURE

实验过程

An assay buffer solution was prepared which was 100 mM HEPES pH 8.0, 10 mM ATP, 2 mM MgCl2, 5 mM DTT, 0.5 mM PMSF. A fructosyl-spermine stock solution was prepared which was 2 mM fructosyl-spermine HCl. A spermine control solution was prepared which was 2 mM spermine HCl.

WORKUP

后处理

  1. customAn assay buffer solution was prepared which