反应详情

温度

60 °C

To prepare pasc#9, a solution of 18 (6.6 mg, 14 μmol) in dry tetrahydrofuran (200 μl) was treated with a mixture of LiOH (0.3 mg, 12 μmol) and water (80 μl) in 1,4-dioxane (400 μl). After stirring at 60° C. for 5 minutes, the solution was acidified with glacial acetic acid and concentrated in vacuo. HPLC purification (see Methods) of the crude reaction mixture afforded 4-(((R)-2-(((R)-4-(((2R,3R,5R,6S)-3,5-dihydroxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)pentanoyl)oxy)-2-phenylethyl)amino)-4-oxobutanoic acid (pasc#9) (1.8 mg, 4 μmol, 29%) as a colorless oil.

1. customTo prepare pasc#9
2. concentrationconcentrated in vacuo
3. customHPLC purification (see Methods) of the crude
4. customreaction mixture