反应详情

3-(Bromomethyl)-4-phenyl-1H-isochromen-1-one (Intermediate C1, 35 mg, 0.111 mmol), 9H-purine-6-thiol hydrate (18.9 mg, 0.111 mmol) and K2CO3 (15.35 mg, 0.111 mmol) were dissolved in DMF (1 ml) and stirred at RT for 1 hrs 30 min. The reaction mixture was then diluted with water (10 ml) and 0.1 N HClaqueous (1 ml). The mixture was extracted with EtOAc and the collected organic phases were dried over Na2SO4, filtered and concentrated under reduced pressure. The crude was purified by Preparative HPLC (method 2) to give the title compound (24 mg, 56%).

1. extractionThe mixture was extracted with EtOAc
2. dry with materialthe collected organic phases were dried over Na2SO4
3. filtrationfiltered
4. concentrationconcentrated under reduced pressure
5. customThe crude was purified by Preparative HPLC (method 2)