反应详情

To a mixture of 5-(2-nitro-ethyl)-2-(pyridin-3-yloxy)pyridine (157.0 mg, 0.64 mmol) described in Manufacturing Example 100-1-2 and methanol (6 mL) was added lithium methoxide (48.7 mg, 1.28 mmol) at room temperature, which was stirred for 1 hour. The reaction mixture was then concentrated under a reduced pressure, which gave a white solid. To a mixture of this solid in dichloromethane (4 mL) and tetrahydrofuran (2 mL) was added titanium tetrachloride (155.0 μL, 1.41 mmol) under nitrogen atmosphere at −78° C., which was stirred for 3 hours at 0° C. Water was added to the reaction mixture, which was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated aqueous sodium chloride, dried over anhydrous magnesium sulfate, and then filtered. The filtrate was concentrated under a reduced pressure. To a mixture of the resulting residue (30.7 mg), 3-ethynyl-pyridin-2,6-diamine (15.4 mg, 0.12 mmol) described in Manufacturing Example 13-1-3, tetrahydrofuran (1 mL), and dimethyl sulfoxide (1 mL) was added triethylamine (32.4 μL, 0.23 mmol) at room temperature, which was stirred for 1 hour at 55° C. The reaction mixture was cooled to room temperature, water was added, and the mixture was extracted with ethyl acetate. The organic layer was separated, washed with water and saturated aqueous sodium chloride, dried over anhydrous magnesium sulfate, and filtered. The filtrate was concentrated under a reduced pressure. The residue thus obtained was purified by reverse-phase high performance liquid chromatography (using an acetonitrile-water mobile phase containing 0.1% trifluoroacetic acid), and then further purified by preparative thin-layer chromatography (NH silica gel, ethyl acetate) to obtain the title compound (3.6 mg, 9%).

1. concentrationThe reaction mixture was then concentrated under a reduced pressure, which
2. customgave a white solid
3. stirringwhich was stirred for 3 hours at 0° C
4. extractionwas extracted with ethyl acetate
5. customThe organic layer was separated
6. washwashed with water and saturated aqueous sodium chloride
7. dry with materialdried over anhydrous magnesium sulfate
8. filtrationfiltered
9. concentrationThe filtrate was concentrated under a reduced pressure
10. stirringwhich was stirred for 1 hour at 55° C
11. extractionthe mixture was extracted with ethyl acetate
12. customThe organic layer was separated
13. washwashed with water and saturated aqueous sodium chloride
14. dry with materialdried over anhydrous magnesium sulfate
15. filtrationfiltered
16. concentrationThe filtrate was concentrated under a reduced pressure
17. customThe residue thus obtained
18. customwas purified by reverse-phase high performance liquid chromatography (
19. additioncontaining 0.1% trifluoroacetic acid), and
20. customthen further purified by preparative thin-layer chromatography (NH silica gel, ethyl acetate)