Acetic Acid → 5-Bromo-6-fluoro-3-(tetrahydro-2H-thiopyran-4-yl)-1H-indole-7-carboxamide

Temperature

90 °C

Tetrahydro-4H-thiopyran-4-one (285 mg, 2.451 mmol) was added to a 2 dram vial containing H3PO4 (2 g, 20.41 mmol) and AcOH (3 mL, 52.5 mmol). The reaction was heated to 90° C. (bath temp), and 5-Bromo-6-fluoro-1H-indole-7-carboxamide (210 mg, 0.817 mmol) was added in 3 portions over 3 min. The reaction was heated at 90° C. for 17. The reaction mixture was cooled to room temperature and was then added slowly to a mixture of ice and saturated NH4OH (10 mL) with stirring. EtOAc (10 mL) was then added, the mixture was filtered, and the precipitate was washed with EtOAc (3×10 mL). The layers of the filtrate were separated, and the aqueous layer was extracted with EtOAc (4×10 mL). The combined organic layers were dried (Na2SO4), filtered, and concentrated under a stream of nitrogen at 50° C., giving 281 mg of crude product. The crude product was dissolved in 1:1 MeOH/DCM, and Isolute was added. The mixture was concentrated under reduced pressure, loaded onto a silica cartridge (12 g), and purified on a Combiflash Companion, eluting at 20 mL/min with a gradient running from 20% EtOAc/hexanes to 70% EtOAc/hexanes over 45 min. The desired fractions were concentrated under reduced pressure and dried under high vacuum, giving 53 mg (17%) of the title compound.

1. additionwas added in 3 portions over 3 min
2. temperatureThe reaction mixture was cooled to room temperature
3. filtrationthe mixture was filtered
4. washthe precipitate was washed with EtOAc (3×10 mL)
5. customThe layers of the filtrate were separated
6. extractionthe aqueous layer was extracted with EtOAc (4×10 mL)
7. dry with materialThe combined organic layers were dried (Na2SO4)
8. filtrationfiltered
9. concentrationconcentrated under a stream of nitrogen at 50° C.
10. customgiving 281 mg of crude product
11. additionwas added
12. concentrationThe mixture was concentrated under reduced pressure
13. custompurified on a Combiflash Companion
14. washeluting at 20 mL/min with a gradient
15. concentrationThe desired fractions were concentrated under reduced pressure
16. customdried under high vacuum

ORD record

ord-45e93d528f454b6fbddef403e0de0aef

Source dataset

uspto-grants-2011_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08071584B2