反应详情

To 50 mg of Rho575 in dimethylformamide, 53 μl of N,N′-diisopropylethylamine was added followed by 78 mg of p-nitrobenzyl chloroformate dissolved in dimethylformamide. The reaction mixture was stirred for 3 days and the solvent was removed in vacuo. The residue was dissolved in chloroform and purified on a Biotage SP4 System with a SNAP 25 g column and a chloroform methanol gradient, to give a pink product.

1. customthe solvent was removed in vacuo
2. dissolutionThe residue was dissolved in chloroform
3. custompurified on a Biotage SP4 System with a SNAP 25 g column
4. customa chloroform methanol gradient, to give a pink product