反应详情

Ethyl β-[[3-[4-(aminoiminomethyl)benzyl]aminobenzoyl]amino]3-pyridine-propanoate (0.35 g; 0.8 mmoles) was treated with 1N lithium hydroxide and methanol (1:1; 20 ml) for 5 min. Methanol was removed under reduced pressure and the residue was purified by HPLC using acetonitrile/water/trifluoroacetic acid system. The desired fractions were collected and lyophilized to give 290 mg of white material. FAB-MS: MH+ =418.0.

1. customMethanol was removed under reduced pressure
2. customthe residue was purified by HPLC
3. customThe desired fractions were collected