反应详情

3-(3-fluoro-5-methoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (BA75, 100 mg, 0.386 mmol) was dissolved in DMF (10 ml). K2CO3 (250 mg, 1.54 mmol) was added and reaction was stirred at room temperature under an argon atmosphere. Iodocyclopentane (0.134 ml, 1.16 mmol) was added with a syringe and reaction was stirred for 2 hours. Solid K2CO3 was removed by filtration. Solvent was partially removed in vacuo. Sodium citrate (50 ml) was added and reaction was extracted with EtOAc. Organic phases concentrated in vacuo and purified using by RP-HPLC (MeCN:H2O:0.1% TFA) to yield BA79.

1. customreaction
2. customreaction
3. stirringwas stirred for 2 hours
4. customSolid K2CO3 was removed by filtration
5. customSolvent was partially removed in vacuo
6. additionSodium citrate (50 ml) was added
7. customreaction
8. extractionwas extracted with EtOAc
9. concentrationOrganic phases concentrated in vacuo
10. custompurified
11. customto yield BA79