Glycine → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Temperature

28 °C

The BIBB 10 medium was adjusted to pH 7.0 with 10% sodium hydroxide solution before sterilization. The culture medium was shaken on a rotary shaker at 260 rpm for 24 hours at 28° C., then 3 ml of same were inoculated into 100 ml of fresh BIBB 10 medium supplemented with 0.5% of glycine before inoculation. These cultures were cultivated for 18 hours under the above cultural environment, and after checking the cells were collected by centrifugation (3500 rpm, 10 min.). The mycelium derived from 100 ml of the culture broth was suspended in 4 ml of PH mixture (pH 7.0) containing the following components:

1. waitThese cultures were cultivated for 18 hours under the above cultural environment
2. customwere collected by centrifugation (3500 rpm, 10 min.)
3. additioncontaining the following components

ORD record

ord-6dd426216b2947d0b4126720468d4ffd

Source dataset

uspto-grants-1996_09

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05552299