反应详情

Into a shaking flask having a volume of 500 ml was charged 100 ml of a medium (pH 7.0) comprising 2% of DL-lysine monohydrochloride, 0.2% of ammonium sulfate, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate and 0.02% of a yeast extract, and the medium was sterilized at 120° C. for 10 minutes. A loopful of Yarrowia lipolytica IFO 1209 was inoculated into the medium, and cultured at 30° C. for 168 hours with shaking. The cell was removed by centrifuging 1000 ml of the above culture broth to obtain a supernatant. After the supernatant was adjusted to have pH 6.0 with hydrochloric acid, ultrafiltration was carried out in order to remove protein and others, whereby a filtrate was obtained. After activated carbon was added to the filtrate to effect decolorization, the filtrate was concentrated under reduced pressure, and 200 ml of ethanol was added to 20 g of the concentrate to obtain 5.8 g of D-lysine monohydrochloride as crude crystals. 5.8 ml of water was added to the crude crystals, and the crude crystals were dissolved by heating, and then recrystallized by cooling to obtain 2.9 g of crystals of D-lysine monohydrochloride.

1. customInto a shaking flask having
2. additiona volume of 500 ml was charged 100 ml of a medium (pH 7.0)
3. extractionextract
4. waitcultured at 30° C. for 168 hours
5. customThe cell was removed
6. customto obtain a supernatant
7. customto remove protein and others, whereby a filtrate
8. customwas obtained
9. additionAfter activated carbon was added to the filtrate
10. concentrationthe filtrate was concentrated under reduced pressure, and 200 ml of ethanol
11. additionwas added to 20 g of the
12. concentrationconcentrate