Sulfuric acid magnesium salt (1:1) → D-(-)-Fructose

HRID 841860Notes

Reaction equation

HRID 841860 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].[O-]S([O-])(=O)=O.[Mg+2].C(O)C(N)(CO)CO.C([O-])(=O)/C=C\C([O-])=O>>[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4]

Participants

4

Reactant 3

HCID 24083Sulfuric acid magnesium salt (1:1)HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 86757951HCID 86757951, compound

Product 1

HCID 5984D-(-)-Fructose

Conditions

Temperature
85 °C

Procedure

The conditions used for the bioconversion are the following: To a 5 ml solution of 0.8 M glucose, 10 mM MgSO4, 1 mM CoCl2, 50 mM Tris/maleate pH 6.4 was added 200 μl of the enzyme preparation. The temperature is maintained at 85° C. for 120 min to effectuate the bioconversion. Subsequently, the amount of fructose formed is determined chromatographically by HPLC.

Workup

  1. additionwas added
  2. custom200 μl of the enzyme preparation

Sources & evidence

ORD record
ord-5f6b9844e25f4184bbc3705199a4481a
Source dataset
uspto-grants-2002_04
Patent number
US06372476B1

Reaction notes

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