HRID8515

反应详情

EQUATION

反应方程式

REACTANTS

反应物

HCID784

Hydrogen peroxide

H2O2

结构：O=C(O[C@@H]1Cc2c(O)cc(O)cc2O[C@@H]1c1cc(O)c(O)c(O)c1)c1cc(O)c(O)c(O)c1

HCID65064

(-)-Epigallocatechin gallate

C22H18O11

PRODUCTS

生成物

结构：O=C(O[C@H]1Cc2c(O)cc(O)cc2O[C@H]1c1cc(O)c(O)c2c(O)c(=O)cc([C@H]3Oc4cc(O)cc(O)c4C[C@H]3O)cc12)c1cc(O)c(O)c(O)c1

HCID102115506

未命名化合物

收率 28.1%

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID180

Acetone

C3H6O

PROCEDURE

实验过程

EC (1 g) and EGCG (1 g) were dissolved in a mixture of acetone-pH 5.0 phosphate citrate buffer (1:10, v/v, 50 mL), which contained 4 mg horseradish peroxidase. While being stirred, 2.0 ml of 3.13% H2O2 was added four times during 45 minutes. The reaction mixture was extracted by ethyl acetate (50 ml×3). After concentration, the residue was subjected to Sephadex LH 20 column eluted with acetone-water solvent system (45%). 220 mg theaflavin 3-gallate was obtained.

WORKUP

后处理

extractionThe reaction mixture was extracted by ethyl acetate (50 ml×3)
concentrationAfter concentration
washeluted with acetone-water solvent system (45%)

来源与证据

来源类型: Open Reaction Database
ORD 记录: ord-8b2708b9760e412ba365ccc68af26b1b
来源数据集: uspto-grants-2006_08
DOI: 10.6084/m9.figshare.5104873.v1
专利: US07087790B2
来源说明: uspto-grants-2006_08

反应 SMILES

[CH2:1]1[C:10]2[C:5](=[CH:6][C:7]([OH:12])=[CH:8][C:9]=2[OH:11])[O:4][C@H:3]([C:13]2[CH:18]=[C:17](O)[C:16]([OH:20])=[C:15]([OH:21])[CH:14]=2)[C@@H:2]1[O:22][C:23]([C:25]1[CH:30]=[C:29]([OH:31])[C:28]([OH:32])=[C:27]([OH:33])[CH:26]=1)=[O:24].OO>CC(C)=O>[CH2:1]1[C:10]2[C:5](=[CH:6][C:7]([OH:12])=[CH:8][C:9]=2[OH:11])[O:4][C@H:3]([C:13]2[CH:18]=[C:18]3[C:13]([C@@H:3]4[O:4][C:5]5[C:10](=[C:9]([OH:11])[CH:8]=[C:7]([OH:12])[CH:6]=5)[CH2:1][C@@H:2]4[O:22][C:23]([C:25]4[CH:26]=[C:27]([OH:33])[C:28]([OH:32])=[C:29]([OH:31])[CH:30]=4)=[O:24])=[CH:14][C:15]([OH:21])=[C:16]([OH:20])[C:17]3=[C:16]([OH:20])[C:15](=[O:21])[CH:14]=2)[C@@H:2]1[OH:22]