反应详情

A solution of Boc-Val-Pro-OH (94.5 mg, 0.30 mmol) in dimethylformamide (1.5 mL), was successively treated, at room temperature, with 1-hydroxibenzotriazol (40.5 mg, 0.30 mmol), N,N-diisopropylcarbodiimide (46.7 μL, 0.30 mmol) and Ara-C (60.9 mg, 0.25 mmol). The stirring was continued until complete disappearance of the starting material (overnight stirring). Then, the solvent was evaporated, the residue was dissolved in ethyl acetate and washed with citric acid (10%), NaHCO3 (10%) and brine. The organic layer was dried (Na2SO4) and evaporated to give a residue that was purified by CCTLC on the chromatotron with dichloromethane:methanol (20:1) to yield Ara-C-dipeptide (A) (21% yield)

1. customThen, the solvent was evaporated
2. dissolutionthe residue was dissolved in ethyl acetate
3. washwashed with citric acid (10%), NaHCO3 (10%) and brine
4. dry with materialThe organic layer was dried (Na2SO4)
5. customevaporated
6. customto give a residue that
7. customwas purified by CCTLC on the chromatotron with dichloromethane:methanol (20:1)