反应详情

To a solution of 8-(3-(S)-amino-3-phenylpropyl)-3-(4-bromobenzyl)-1,3,8-triaza-spiro[4.5]decane-2,4-dione (60 μmol) in anhydrous DCE (1 mL) was added triethylamine (10 μL, 72 μmol) and a solution of methyl chloroformate (72 μmol) in anhydrous DCE. The reaction mixture was agitated overnight at room temperature and then concentrated in vacuo. The residue was purified by semi-preparative HPLC (Method D) and lyophilized to give Compound 82 as a colorless solid (14.1 mg, 42%).

1. concentrationconcentrated in vacuo
2. customThe residue was purified by semi-preparative HPLC (Method D)