Sodium Chloride → Atrolactic acid
Reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9]O)O)O)[OH:4].O=[CH:12][C@@H:13]([C@H:15]([C@@H]([C@@H](CO)O)O)O)O.[Na+].[Cl-].Cl.N[C@H](C(O)=O)CS.[C:33](=[O:36])([O-])[O-:34].[Ca+2]>>[C:5]1([C:3]([CH3:2])([OH:4])[C:33]([OH:34])=[O:36])[CH:15]=[CH:13][CH:12]=[CH:9][CH:7]=1Participants
Conditions
- Temperature
- 30 °C
Procedure
25 parts by weight and 180 parts by weight of these xylose and glucose, respectively, were mixed with 10,000 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 100 parts by weight of a pre-culture fluid of phenyllactic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 6 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by purification to obtain 9 parts by weight of phenyllactic acid.
Workup
- customthe mixture formed
- additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
- additionThereafter, the culture medium treated
Sources & evidence
- ORD record
- ord-39159b9e913d4c2697913585d152d864
- Source dataset
- uspto-grants-2007_09
- Patent number
- US07273734B2
Reaction notes
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