Sodium Chloride → HCID 86587262, compound

HRID 917280Notes

Reaction equation

HRID 917280 reaction equation
O=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([CH2:9]O)O)O)[OH:4].[O:11]=[CH:12][C@@H:13]([C@H:15]([C@@H]([C@@H](CO)O)O)O)O.[Na+].[Cl-].Cl.N[C@H:27]([C:30]([OH:32])=[O:31])[CH2:28]S.[C:33](=[O:36])([O-])[O-:34].[Ca+2]>>[C:5]1([C:3]([CH3:2])([OH:4])[C:33]([OH:34])=[O:36])[CH:15]=[CH:13][CH:12]=[CH:9][CH:7]=1.[OH:11][CH:27]([CH2:28][CH:3]([CH3:5])[CH3:2])[C:30]([OH:32])=[O:31]

Participants

6

Reactant 5

HCID 5234Sodium ChlorideHCID 10112Calcium CarbonateHCID 60960Cysteine HydrochlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 644160aldehydo-D-Xylose

Product 1

HCID 86587262HCID 86587262, compound

Conditions

Temperature
30 °C

Procedure

25 parts by weight and 180 parts by weight of these xylose and glucose, respectively, were mixed with 10,000 parts by weight of a culture medium (10 g/l of peptone, 5 g/l of yeast extract, 2 g/l of meat extract, 5 g/l of NaCl, 2 g/l of cysteine hydrochloride and 5 g/l of calcium carbonate), and the mixture formed was injected into a pressure bottle. After the gaseous-phase portion in the bottle was displaced with nitrogen gas, the bottle was hermetically closed with a butyl rubber stopper, which was then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes). Thereafter, the culture medium treated was cooled to 30° C., and 100 parts by weight of a pre-culture fluid of phenyllactic acid/2-hydroxyisocaproic acid productive strain HICA432 isolated in the manner as described previously was added thereto by means of a syringe when the bottle was kept hermetically stoppered, followed by a static culture at 30° C. for 6 days. Thereafter, the culture fluid was filtered, and the water and volatile components of the filtrate obtained were evaporated off under normal-temperature reduced pressure, followed by NMR analysis. As a result, it was ascertained that a mixture of 8.5 parts by weight of phenyllactic acid and 8.0 parts by weight of 2-hydroxyisocaproic acid was obtained.

Workup

  1. customthe mixture formed
  2. additionwas then treated in an autoclave (121° C., 98 kPa pressure, 10 minutes)
  3. additionThereafter, the culture medium treated

Sources & evidence

ORD record
ord-e9aadaf581514fc1b20b5861a0f6c8c6
Source dataset
uspto-grants-2007_09
Patent number
US07273734B2

Reaction notes

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