反应详情

To 30 ml of a ethyl acetate solution of compound (4) prepared from 2.12 g of compound (3) by a known method was added 5 ml of a DMF solution of 1.17 g of compound (100) under ice-cooling and the reaction was maintained overnight in a refrigerator. The reaction mixture was concentrated and the residue thus formed was subjected to silica gel column chromatography. By eluting the product with chloroform-methanol-aqueous ammonia (90:10:1), 2.00 g of Nα -benzyloxycarbonyl-L-histidyl-N-[3-(2-oxo-1-pyrrolidinyl)-propyl]-L-prolinamide (101) was obtained.

1. temperaturecooling
2. temperaturethe reaction was maintained overnight in a refrigerator
3. concentrationThe reaction mixture was concentrated
4. customthe residue thus formed
5. washBy eluting the product with chloroform-methanol-aqueous ammonia (90:10:1)