Ammonia → L-Phenylalanine

A microorganism was grown in the same manner as described in Section 11 above. From 30 g of the collected and washed cells, L-phenylalanine ammonia-lyase was extracted and purified according to the same procedure as described in Section 11 above. The purified L-phenylalanine ammonia-lyase was dissolved in 20 ml of a NH4OH—(NH4)2SO4 buffer solution (pH 10.0) containing 8M NH The resulting solution was placed in a dialysis tube (a product of Union Carbide Corp.), which was sealed at both ends. This tube was immersed in a reaction mixture prepared by adding 4 g of cinnamic acid to 200 ml of a NH4OH—(NH4)2SO4 buffer solution (pH 10.0) containing 8M NH3. The reaction was carried out at 30° C. for 40 hours with gentle stirring of the outer liquid. After that time, the procedure of Section 13 was repeated to obtain 2.0 g of L-phenylalanine.

1. customFrom 30 g of the collected
2. washwashed cells, L-phenylalanine ammonia-lyase
3. extractionwas extracted
4. custompurified
5. dissolutionThe purified L-phenylalanine ammonia-lyase was dissolved in 20 ml of a NH4OH—(NH4)2SO4 buffer solution (pH 10.0)
6. additioncontaining 8M NH The resulting solution
7. customwas placed in a dialysis tube
8. custom), which was sealed at both ends
9. customThis tube was immersed in a reaction mixture
10. customprepared

ORD record

ord-d8878d8a4f2c43e2afd91954f99adce0

Source dataset

uspto-grants-2002_10

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06472196B1