Reaktionsgleichung
C1C=CC2S(=O)(=O)OC(C3C=C(Br)C(O)=C(Br)C=3)(C3C=C(Br)C(O)=C(Br)C=3)C=2C=1.CN(C1C=CC(C(C2C=CC(N(C)C)=CC=2)CC2C(S(O)(=O)=O)=CC=CC=2)=CC=1)C.C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.CC[N+]1C(C2C=CC=CC=2)=C2C(C=CC(N)=C2)=C2C=1C=C(N)C=C2.[Br-].[B:108]([OH:111])([OH:110])[OH:109].[CH2:112]([N:123]([CH2:128][C:129]([OH:131])=[O:130])[CH2:124][C:125]([OH:127])=[O:126])[CH2:113][N:114]([CH2:119][C:120]([OH:122])=[O:121])[CH2:115][C:116]([OH:118])=[O:117].C(O)C(N)(CO)CO>O>[B:108]([OH:111])([OH:110])[OH:109].[CH2:113]([N:114]([CH2:119][C:120]([OH:122])=[O:121])[CH2:115][C:116]([OH:118])=[O:117])[CH2:112][N:123]([CH2:128][C:129]([OH:131])=[O:130])[CH2:124][C:125]([OH:127])=[O:126]Komponenten
8
Edukt 5
Produkt 2
Reagenzien, Katalysatoren, Lösungsmittel 1
Versuchsvorschrift
The PCR reaction was monitored by agarose gel electrophoresis as follows: A 1.5% agarose (FMC Bioproducts) electrophoresis gel in tris-borate EDTA (“TBE”) 1× buffer (89 mM Tris-borate (Fisher Biotech), 89 mM boric acid (Mallinckrodt) and 2 mM EDTA (Mallinckrodt)) was prepared. Five to ten microliters of PCR products were loaded onto the gel with 1 μl dye 10× (0.5% bromophenol blue (Sigma), 0.5% Xylene cyanol (Gibco-BRL), 40% (w/v) sucrose (IBI) in water). Electrophoresis was run in TBE 1× at 120 V for 45 minutes. The gel was stained with ethidium bromide (10 μg/ml, Sigma) for 5 minutes. PCR products were visualized with ultraviolet light.
Aufarbeitung
- customThe PCR reaction
- customfor 45 minutes
Quellen und Nachweise
- ORD-Eintrag
- ord-aeeabda2c02645fc96178ef2414a9773
- Quelldatensatz
- uspto-grants-2001_06
- Patentnummer
- US06242182B1
Verknüpfte Notizen
Wird geladen…