L-(-)-Sorbose → Sorbosone, L-

HRID 2073159

Reaktionsgleichung

Reaktionsgleichung von HRID 2073159
[OH:1][CH2:2][C:3]([C@H:5]([C@@H:7]([C@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])=[O:4].P([O-])([O-])([O-])=O.[K+].[K+].[K+]>C[N+]1C2C(=CC=CC=2)N=C2C=1C=CC=C2.COS([O-])(=O)=O>[CH2:11]([OH:12])[C@H:9]([OH:10])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C:3]([CH:2]=[O:1])=[O:4]

Komponenten

4

Edukt 2

HCID 6904L-(-)-Sorbose3×HCID 62657Potassium Phosphate, Tribasic

Produkt 1

HCID 10921067Sorbosone, L-

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 9285Phenazine MethosulfateLösungsmittel

Versuchsvorschrift

The reaction mixture containing 400 ml of purified membrane-bound L-sorbose dehydrogenase (total activity, 120 units), as prepared by the manner as described in steps (1) to (5) of Example 1, 50 ml of 0.5M potassium phosphate buffer (pH 6.0). 50 ml of 1M L-sorbose solution and 10 ml of 0.2M phenazine methosulfate solution was incubated at 30° C. with gentle shaking. As a result, L-sorbosone was formed with the rate of 224 mg/hr.

Aufarbeitung

  1. customas prepared by the manner
  2. customwas incubated at 30° C. with gentle shaking

Quellen und Nachweise

ORD-Eintrag
ord-9f0bf67697f44edd8787cd0b3876adcd
Quelldatensatz
uspto-grants-1990_04
Patentnummer
US04916069

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