Staurosporine → Biphenyl

Reaktionsgleichung

CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4.O=Cc1nc2ccccc2s1>CC(=O)N(C)C.CC(=O)O.CC(=O)O[BH-](OC(C)=O)OC(C)=O.CC(C)[O-].CC(C)[O-].CC(C)[O-].CC(C)[O-].[Na+].[Ti+4]>CO[C@@H]1[C@H](N(C)Cc2nc3ccccc3s2)C[C@H]2O[C@]1(C)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4

Komponenten

Reaktionsbedingungen

Temperatur: 22 °C

Aufarbeitung

additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
additionThe reactions were diluted with 3x1 uL DMSO (containing PhPh as an internal standard) using the Mosquito(R). The reactions were mixed after each dispense operation.
aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

Quellen und Nachweise

ORD-Eintrag: ord-3891bc09a5f544cbb002999a1e653e2a
Quelldatensatz: Cernak miniaturized reductive amination
DOI: 10.1038/s44160-023-00351-1
Originallink: Quelle ansehen

Notizen zur Reaktion

Wird geladen…

Komponenten

Edukt 2

HCID 44259Staurosporine

HCID 2416081,3-Benzothiazole-2-carbaldehyde

Produkt 4

HCID 7095Biphenyl

HCID 44259Staurosporine

HCID 268122(1,3-Benzothiazol-2-yl)methanol

HCID 178496698HCID 178496698, Verbindung

Reagenzien, Katalysatoren, Lösungsmittel 4

HCID 176Acetic AcidReagenz

HCID 11026Titanium isopropoxideReagenz

HCID 5049666Sodium triacetoxyborohydrideReagenz

HCID 31374DimethylacetamideLösungsmittel7×

Reaktionsbedingungen

Temperatur: 22 °C

Aufarbeitung

additionThe next morning, the Mosquito(R) was used to dilute the 100 mM reactions to 50 mM via addition of 1 uL DMSO to each reaction well, followed by dispense mixing.
additionThe reactions were diluted with 3x1 uL DMSO (containing PhPh as an internal standard) using the Mosquito(R). The reactions were mixed after each dispense operation.
aliquotMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.
additionMosquito(R) was used to transfer 1 uL of each quenched reaction from the 1,536-well reaction plate to 384-well analytical plates containing 60 uL DMSO for analysis by UPLC.

Quellen und Nachweise

ORD-Eintrag: ord-3891bc09a5f544cbb002999a1e653e2a
Quelldatensatz: Cernak miniaturized reductive amination
DOI: 10.1038/s44160-023-00351-1
Originallink: Quelle ansehen

Notizen zur Reaktion

Wird geladen…