C(O)[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O.[NH2:24][C@H:25]([C:27]([OH:29])=[O:28])[CH3:26].N[C@H](C(O)=O)[CH2:32][C:33](=[O:35])[NH2:34].N[C@H](C(O)=O)CC(O)=O.N1CCC[C@H]1C(O)=O.N[C@H](C(O)=O)CO>[Cl-].OCC[N+](C)(C)C>[NH2:24][C@H:25]([C:27]([OH:29])=[O:28])[CH2:26][CH2:32][C:33](=[O:35])[NH2:34]The defined oral diets contained 427 calories/100 g and included 0.2% (w/w) choline chloride, 10% corn oil, 46.9% dextrin-white, 23.4% sucrose, 5% salt mixture, 0.5% vitamin mixture. Both diets consisted of 14% amino acids, including 7.17% total essential amino acids and 6.83% total non-essential amino acids. The diet lacking GLN (-GLN) contained 0.937% each of alanine, asparagine, aspartic acid, proline and serine. The GLN-enriched diet (+GLN) contained 0.237% of these amino acids and 3.5% GLN. Thus, GLN represented 25% of total amino acids and 51% of non-essential amino acids in the +GLN diet.
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